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磷酸化蛋白激酶C α/β2重組兔單克隆抗體
  • 產(chǎn)品貨號：
    BN42101R
  • 中文名稱：
    磷酸化蛋白激酶C α/β2重組兔單克隆抗體
  • 英文名稱：
    Rabbit anti-Phospho-PKC alpha (Thr638) Monoclonal antibody
  • 品牌：
    Biorigin

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  • BN42101R-50ul

    50ul

    ￥2020.00

    交叉反應(yīng)：Human,Mouse,Rat(predicted:Chicken,Pig,Cow,Horse) 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt

  • BN42101R-100ul

    100ul

    ￥3240.00

    交叉反應(yīng)：Human,Mouse,Rat(predicted:Chicken,Pig,Cow,Horse) 推薦應(yīng)用：WB,IHC-P,IHC-F,ICC,IF,Flow-Cyt

產(chǎn)品描述

英文名稱Phospho-PKC alpha (Thr638)
中文名稱磷酸化蛋白激酶C α/β2重組兔單克隆抗體
別    名PKC alpha(T638); PKC(Phospho-Thr641); Phospho-PKC alpha/beta II (Thr638/641); Phospho-PKC alpha/beta II (Thr638 + 641); PKC alpha; AAG6; Aging associated gene 6; PICK 1; PKC A; PKC alpha; PKCA; PRKACA; PRKC A; PRKCA; Protein Kinase C alpha; Protein kinase C alpha type; KPCB_HUMAN; PKC α.  
產(chǎn)品類型磷酸化抗體 
研究領(lǐng)域腫瘤  免疫學(xué)  信號轉(zhuǎn)導(dǎo)  細(xì)胞凋亡  轉(zhuǎn)錄調(diào)節(jié)因子  激酶和磷酸酶  
抗體來源Rabbit
克隆類型Monoclonal
克 隆 號4B3
交叉反應(yīng)Human, Mouse, Rat,  (predicted: Chicken, Pig, Cow, Horse, )
產(chǎn)品應(yīng)用WB=1:500-2000 IHC-P=1:50-1:500 IHC-F=1:50-1:500 Flow-Cyt=2ug/Test ICC=1:50 IF=1:50-1:500 （石蠟切片需做抗原修復(fù)）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量77kDa
細(xì)胞定位細(xì)胞核 細(xì)胞漿 細(xì)胞膜 
性    狀Liquid
濃    度1mg/ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human PKC alpha around the phosphorylation site of Thr638:VL(p-T)PP 
亞    型IgG
純化方法affinity purified by Protein A
儲 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
產(chǎn)品介紹Protein Kinase c alpha (PKC alpha) is an 77 kDa member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/ threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation. PKC alpha is an ubiquitously expressed PKC isozyme that has been implicated in the regulation of a broad range of cellular functions including proliferation, differentiation, development, migration, cell cell adhesion, cell extracellular matrix adhesion, and solute transport. The activation loop threonine (threonine 497 in PKC alpha) of conventional PKCs is phosphorylated by phosphoinositide dependent kinase 1 (PDK1). This phosphorylation is necessary for the autophosphorylation of threonine 638 in the carboxy terminus of PKC alpha, a step that is critical for regulating the rate of PKC alpha dephosphorylation and inactivation.

Function:
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. May participate in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis.

Subunit:
Recruited in a circadian manner into a nuclear complex which also includes BMAL1 and GNB2L1/RACK1 (By similarity). Interacts with ADAP1/CENTA1, CSPG4 and PRKCABP. Binds to SDPR in the presence of phosphatidylserine. Interacts with PICK1 (via PDZ domain). Interacts with TRIM41.

Subcellular Location:
Cytoplasm. Cell membrane; Peripheral membrane protein. Mitochondrion membrane; Peripheral membrane protein (Probable). Nucleus.

Post-translational modifications:
Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity. Phosphorylation at Tyr-662 by SYK induces binding with GRB2 and contributes to the activation of MAPK/ERK signaling cascade.

Similarity:
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain.

SWISS:
P17252

Gene ID:
5578

Database links:

Entrez Gene: 5578 Human

Entrez Gene: 18750 Mouse

Entrez Gene: 24680 Rat

Omim: 176960 Human

SwissProt: P17252 Human

SwissProt: P20444 Mouse

SwissProt: P05696 Rat

Unigene: 531704 Human

Unigene: 708867 Human

Unigene: 222178 Mouse

Unigene: 207908 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.